Berfin Doğan, Oytun Erbaş

Institute of Experimental Medicine, Gebze-Kocaeli, Türkiye

Keywords: Cryopreservation, slow-freezing, stem cell banking, stem cells, vitrification.


Cryopreservation and stem cell banking have become increasingly popular topics in recent years. The purpose of cryopreservation is to store frozen cells or tissues with minimal damage to their structures and functions. With the ability to freeze and store mammalian embryos, the complete genomes of different species can be preserved for extended periods. People wish to take precautions for potential future health issues for themselves or their family members. When selecting a bank for collecting stem cells, several factors need to be considered. These factors include quality control and assurance protocols, the donor selection process, storage methods, compliance with international standards, and customer services. It is important to examine these factors before registering with a stem cell bank. Cryopreservation is a process of storing human tissues and cells at very low temperatures. This method is used to create a backup plan for using these cells in case they are somehow damaged. Cryopreservation technology is employed to preserve tissues, cells, and organs for an extended period in case of freezing or other types of damage. Stem cell banks, on the other hand, can be a crucial resource for individuals looking to reduce the impact of a disease or undergo stem cell therapy. Stem cells can assist in repairing damaged tissues and, in some cases, even aid in the treatment of diseases like cancer. Additionally, the storage conditions of stem cells are of critical importance. Stem cells must be protected from factors such as temperature, humidity, and other variables, and should be kept away from factors that may cause changes during the storage period. This review discusses cryopreservation and stem cell banking.

Cite this article as: Doğan B, Erbaş O. Fundamental principles of cryopreservation and stem cell banking. D J Tx Sci 2023;8(1-2):68-73. doi: 10.5606/dsufnjt.2023.11.

Author Contributions

All authors contributed equally to the article.

Data Sharing Statement:
The data that support the findings of this study are available from the corresponding author upon reasonable request.

Conflict of Interest

The authors declared no conflicts of interest with respect to the authorship and/ or publication of this article.

Financial Disclosure

The authors received no financial support for the research and/or authorship of this article.